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Additional file 6: Influence of r-tPA on MPO and NE. PBMCs of healthy donors were incubated with 0.5 or 1 μg/ml r-tPA for 4 h at 37 °C in 5% CO 2 . Afterwards, MPO and NE were measured intracellularly in granulocytes by flow cytometry (A, C), expressed in MFI, and in the supernatant using ELISA (B, D), expressed in ng/ml (n = 10). *p 0.01 ... mpo slot pulsa